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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
- IIT JAM
- Biotechnology (BT)
Explain the steps of hat selection
explain the steps of HAT selection #monoclonal antibody#hybridomas
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Krishan k jakhad Best Answer
HAT Medium (hypoxanthine-aminopterin-thymidine medium) is a selection medium for mammalian cell culture, which relies on the combination of aminopterin, a drug that acts as a powerful folate metabolism inhibitor by inhibiting dihydrofolate reductase, with hypoxanthine (a purine derivative) and thymidine (a deoxynucleoside) which are intermediates in DNA synthesis. The trick is that aminopterin blocks DNA de novo synthesis, which is absolutely required for cell division to proceed, but hypoxanthine and thymidine provide cells with the raw material to evade the blockage (the "salvage pathway"), provided that they have the right enzymes, which means having functioning copies of the genes that encode them. see images