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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
Palak Varma posted an Question
May 27, 2021 • 17:10 pm
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30 points
30 points
- IIT JAM
- Biotechnology (BT)
How does angle of rotation depends on concentration of sample,length of tube, wavelength of light and temperature ?
how does angle of rotation depends on concentration of sample,length of tube, wavelength of light and temperature ?
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Priyanshu kumar![best-answer]()
Optical activity is the ability of a compound to rotate the plane of polarized light. This property arises from an interaction of the electromagnetic radiation of polarized light with the unsymmetric electric fields generated by the electrons in a chiral molecule. The rotation observed will clearly depend on the number of molecules exerting their effect that depends upon the concentrations. Observed rotations are thus converted into specific rotations that are a characteristic of the compounds according to the formula below. Effect of concentration:- The more the sample is condensed (the more molecules per volume unit), the more molecules are encountered. It would have greater optical rotations of condensed solutions and neat tests than dilute solutions. Effect of temperature:- That being said, temperature will influence how the material interacts with light, and there would be no significant difference in precise rotation in most typical solutions at mild temperatures. This optical behaviour affects the temperature. This depends on the light wavelength used and on the material.