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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
Chanchal Kumar posted an Question
February 02, 2021 • 00:16 am
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30 points
30 points
- IIT JAM
- Biotechnology (BT)
I think the answer is b but according to book it is d
does ethanol denatures dsdna can u please clear the confusion
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Anup Padwal![best-answer]()
All Heat denaturation: When a DNA solution is heated enough, the double-stranded DNA unwinds and the hydrogen bonds that hold the two strands together weaken and finally break. ... The temperature at which the DNA strands are half denatured, meaning half double-stranded, half single-stranded, is called the melting temperature(Tm). Denaturation using Urea: Denaturing requires disrupting these hydrogen bonds. The most commonly used DNA denaturants are urea and formamide. Each of these forms hydrogen bonds with the DNA bases, "saturating" H-bond sites and preventing the formation of inter-base bonds. High pH: At high pH, then, the solution is rich in hydroxide ions, and these negatively-charged ions can pull hydrogen ions off of molecules like the base pairs in DNA. This process disrupts the hydrogen bonding that holds the two DNA strands together, causing them to separate. Ethanol: Ethanol is preferred over formalin if specimens will be used for barcoding or other molecular methods because it does not directly affect DNA integrity and, in high enough concentrations (e.g., ≥95%), ethanol denatures proteins that may degrade DNA.