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Ashutosh posted an Question
June 16, 2020 • 01:37 am 30 points
  • IIT JAM
  • Biotechnology (BT)

. in an enzyme catalyzed reaction, doubling the amount of enzyme leads to a change in which of the following?

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  • Abhijeet Gaurav best-answer

    Vmax Will be affected. As Vmax is maximum velocity of enzyme through which it converts the substrates into products. So, Upon doubling the Enzyme Concentration the Vmax Will be also double. So Vmax Will be changed. It Will be doubled because Enzyme Concentration is increased so it Will achieve max velocity in less time & the Vmax Will be doubled . Now Kcat = Vmax/ [E] So, Kcat won't be affected because Vmax and Enzyme Concentration are changed in same proportion. Km Will be unchanged because it is related to substrates Concentration. So, Kcat/Km Will also be unaffected.

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