Nisarg posted an Question
April 15, 2021 • 14:33 pm 30 points
  • IIT JAM
  • Biotechnology (BT)

In case of transcript analysis by pcr why do we amlify our last product?because our goal is to determine the position of the end points or introns, which can be

in case of transcript analysis by PCR why do we amlify our last product?because our goal is to determine the position of the end points or introns, which can be achieved only by comparing only one sequence!

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    Krishan k jakhad best-answer

     Reverse transcriptase is an RNA-dependent DNA polymerase, catalyzing DNA synthesis using RNA as the template. ...thus ur end product of RT-PCR is known as complementary DNA (cDNA). cDNA is not subject to RNase degradation, thus more stable than RNA. In RT-PCR, the starting RNA is subsequently degraded, dsDNA is produced, and PCR amplification (thermal cycler) proceeds in the usual manner. ...to make more and more copies of dsDNA now... In simple words initially you were having RNA.....u made cDNA using RT-PCR or RT-qPCR which is single strand in nature...then u make it dsDNA....then came to ur question ...now PCR is performed to make multiple copies of dsDNA you had.

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