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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
Eduncle Best Answer
"RNA polymerase Il (RNAP Il and Poi Il) is a protein-protein complex. It is one of the three RNAP enzymes found in the nucleus of eukaryotic cells. It catalyzes the transcription of DNA to synthesize precursors of mRNA and most snRNA and microRNA."
Direct evidence that miRNA genes are transcribed by RNA polymerase Il (pol Il). The primary miRNA transcripts (pri-miRNAs) contain cap structures as well as poly(A) tails, which are the unique properties of class Il gene transcripts.
Small interfering RNA (siRNA), sometimes known as short interfering RNA or silencing RNA, is a class of double-stranded RNA molecules, 20-25 base pairs in length, similar to miRNA, and operating within the RNA interference (RNAi) pathway. It interferes with the expression of specific genes with complementary nucleotide sequences by degrading mRNA after transcription, preventing translation.