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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
- IIT JAM
- Biotechnology (BT)
In here, in the 4th point, we will have to use a counterstain of a different colour, given in example is safranin, but safranin and crystal violet are both for
In here, in the 4th point, we will have to use a counterstain of a different colour, given in example is safranin, but safranin and crystal violet are both for gram -ve bacterias.How will two strains which are for the same gram bacteria can be used to distinguish between them. I am confused with it. Explain in brief please.
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Anup Padwal![best-answer]()
Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls and stain react with peptidoglycan forming strong bond which not be easily dissociated. when we wash mixture of gram positive and gram negative bacteria only gram negative bacteria leaves the colour because less stable bond. note important thing that saffranin also react with cell wall component bit when cells already stained with crystal violet stain can not absorb saffranin. Because gram positive bacteria cell wall components are tightly bind with crystal violet.