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Ashutosh posted an Question
January 27, 2021 • 09:47 am 30 points
  • IIT JAM
  • Biotechnology (BT)

In plant tissue culture, differentiation of callus to root requires (a) high auxin and low cytokinin. (b) low auxin and high cytokinin low auxin and low cytokin

In plant tissue culture, differentiation of callus to root requires (a) high auxin and low cytokinin. (b) low auxin and high cytokinin low auxin and low cytokinin (c) (d) high auxin and high cytokinin

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    Yogita Basnal

    option (a) is right answer. For differentiation of callus to roots requires high auxin and low cytokinin. It has been proved experimentally that auxin to cytokinesis ratio impacts morphogenesis. When ipt gene is mutated it leads to high auxin to cytokinin ratio which further causes root differentiation. But when auxin biosynthesis genes are mutated it leads to low auxin to cytokinin ratio which ultimately leads to the proliferation of shoots in the undifferentiated callus. When concentration of auxin to cytokinin is intermediate then neither root morphogenesis occurs nor shoot i. e. the callus remains undifferentiated.

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