Nisarg posted an Question
April 15, 2021 • 20:02 pm 30 points
  • IIT JAM
  • Biotechnology (BT)

In primer extension, why do we correlate the cdna with dna, why not with the rna transcript? because in both the cases we will come to know about the position o

in primer extension, why do we correlate the cDNA with DNA, why not with the RNA transcript? because in both the cases we will come to know about the position of 5' end!

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    Krishan k jakhad best-answer

    bcz cDNA is complementary DNA having T instead of U in RNA but it is made from RNA... via a special enzyme Reverse transcriptase. Reverse transcriptase (RT) is an RNA-dependent DNA polymerase, catalyzing DNA synthesis using RNA as the template. The end product is known as complementary DNA (cDNA). cDNA is not subject to RNase degradation, making it more stable than RNA. In RT-PCR, the starting RNA is subsequently degraded, dsDNA is produced.

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