IIT JAM
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Anjali gupta Asked a Question
June 15, 2021 5:20 pmpts 30 pts
  • IIT JAM
  • Biotechnology (BT)

S'atgcatgcatgcatgcaatrtgctatatagctacactatagacattatatagagagacgagatatatatatgccgcg acagagacagacagacagagagagtatatagagcacaca3 1) design forward and reverse primer fo

S'ATGCATGCATGCATGCAATrTGCTATATAGCTACACTATAGACATTATATAGAGAGACGAGATATATATATGCcGCG ACAGAGACAGACAGACAGAGAGAGTATATAGAGCACACA3 1) Design forward and reverse primer for a bove mentianed DNA sequence. 2) Determine Tm of both Farward and reverse primer. 3) What would be the annealing temperature for such primer pair in a PCR?
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  • Abhijeet Gaurav thankyou
    Forward Primer :- ATGCATGCATGC Reverse Primer :- TGTGTGCTCTAT (nA+nT)*2+(nG+nC)*4 = Tm Forward Primer = 36°C Reverse Primer = 34°C Annealing Temp would be Lower than Tm that is ...
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