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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
- IIT JAM
- Biotechnology (BT)
The following data refer to the hydrolysis of various tripeptides into their n- terminal amino acids and c-terminal dipeptides. substrate kaat (s) kan (mm) a. l
The following data refer to the hydrolysis of various tripeptides into their N- terminal amino acids and C-terminal dipeptides. substrate Kaat (s) Kan (mM) A. L-Pro-Gly-Gly B. L-Leu-Gly-Gly C. L-Ala-Gly-Gly D. L-Ala-L-Ala-L-Ala 385 190 365 1.3 0.55 14 298 0.52 Which substrate (A, B, C, or D) would be hydrolysed most rapidly in the early stages of reaction if a sample of enzyme was added to a mixture of ail four substrates in equimolar concentration ?
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Anup Padwal![best-answer]()
Fastest reaction will be possible when Kcat high and Km low. Kcat high because it is the rate were Product is formed from ES complex. Km low because it is concentration of subtrate to achieve 50% Vmax. When we take the ratio, more the ratio more the speed of reaction.