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Anam Fatma posted an Question
November 23, 2021 • 17:29 pm
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20 points
20 points
- IIT JAM
- Biotechnology (BT)
The relationship between log10 (mw) [where mw = molecular weight in kda of a mixture of protein standards and their retention factors (r) obtained from native-p
The relationship between log10 (MW) [where MW = molecular weight in kDa of a mixture of protein standards and their retention factors (R) obtained from native-PAGEis log10 (MW = -2R+ 3 If the measured log10 retention factor for a protein with 180 amino acids is 0.5, then the number of identical monomers in the protein is n01c)
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Priya sarda![best-answer]()
Here is the solution of your query - Log10 (MW) = -2Rf+ 3 Log10 (MW) = -2*.5 + 3 Log10 (MW) = -1 + 3 MW = 100 kDa MW of 180 amino acids is = 180*110 Da = 19.8 kDa ~ 20kDa So, the number of identical monomers in the protein is = 100/20= 5 Thank you