Savia Asked a Question
September 17, 2020 8:52 pmpts 30 pts
The specific activity of an enzyme in a crude extract of E. coli is 9.5 units/mg of protein. The specific activity increased to 68 units/mg of protein upon ion-exchange chromatography. The fold purification is
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  • Anup padwal thankyou
    Answer is 7.15 fold The first you calculated is protein recovery. In order to determine the enzyme yield you have to calculate total enzyme activity before loading on gel (call thi...
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