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Arindam Paul posted an Question
November 20, 2020 • 21:02 pm 30 points
  • IIT JAM
  • Biotechnology (BT)

The techniques to show that a protein is a homotetramer with a subunit molecular weight of 25 kda are (a) gel filtration and native page (b) affinity and ion ex

The techniques to show that a protein is a homotetramer with a subunit molecular weight of 25 kDa are (A) gel filtration and native PAGE (B) affinity and ion exchange chromatography (C) SDS-PAGE and gel filtration D) isoelectric focusing and SDS-PAGE

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    Anup Padwal best-answer

    Option D it is collectively known as 2D PAGE. 1D: seperated by charge 2D: seperated by mass but the answer is not perferct, we can select it because from given option it is better. Answer should be SDS in presence and absence of Beta mercaptoethanol.

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