Savia Asked a Question
September 17, 2020 10:46 pmpts 30 pts
V ighigilit Shown below is a plasmid vector (P) and an insert (Q). The insert was cloned into the BamHI site of the vector. The recombinant plasmid was isolated and digested with BamHI or Xhol The results from the digestion experiments are shown in (R). BamHI Gkb S kb S kb plasmid BamHI Xhol BamHI 4 kb vector 0.3 kb kb 2 kb 1 kb Insert 1 kb EcoRI P Q R Which one of the following explains the digestion results shown in (R)? (A) The insert did not ligate to the vector. (B) One copy of the insert ligated to the vector. (C) The insert ligated to the vector as two landem copies (D) The insert ligated to the vector as two copies but not in tandem.
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  • Ruby negi
    answer c
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  • Abhijeet Gaurav thankyou
    Answer is (C). According to the results of digestion in Gel it shows that Upon digestion with BamH1 it gives 2 band of 5Kb & 1Kb. So it means that the insert ligated into the pla...
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