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Savia posted an Question
September 18, 2020 • 04:16 am 30 points
  • IIT JAM
  • Biotechnology (BT)

V ighigilit shown below is a plasmid vector (p) and an insert (q). the insert was cloned into the bamhi site of the vector. the recombinant plasmid was isolated

V ighigilit Shown below is a plasmid vector (P) and an insert (Q). The insert was cloned into the BamHI site of the vector. The recombinant plasmid was isolated and digested with BamHI or Xhol The results from the digestion experiments are shown in (R). BamHI Gkb S kb S kb plasmid BamHI Xhol BamHI 4 kb vector 0.3 kb kb 2 kb 1 kb Insert 1 kb EcoRI P Q R Which one of the following explains the digestion results shown in (R)? (A) The insert did not ligate to the vector. (B) One copy of the insert ligated to the vector. (C) The insert ligated to the vector as two landem copies (D) The insert ligated to the vector as two copies but not in tandem.

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  • Abhijeet Gaurav best-answer

    Answer is (C). According to the results of digestion in Gel it shows that Upon digestion with BamH1 it gives 2 band of 5Kb & 1Kb. So it means that the insert ligated into the plasmid vector so it's total length became 6kb which Upon digestion have 2 band. And Digestion with Xho1 show they also have 2 band. If it had not ligated then it would have 3 bands instead of 2. So they ligated & in tandem means adjacent to each other.

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