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Yash Hansraj Pagar posted an Question
September 15, 2021 • 00:24 am
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- IIT JAM
- Biotechnology (BT)
What is isoelectronic point?
what is isoelectronic point?please explain concept in detail (from volume no. 1 page no. 204)
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The isoelectric point (pI) is the pH at which a particular molecule carries no net electrical charge. The net charge on the molecule is affected by the pH of its surrounding environment and can become more positive or negative due to the gain or loss of protons, respectively. The isoelectric point (pI) is the pH of a solution at which the net charge of a protein becomes zero. At solution pH that is above the pI, the surface of the protein is predominantly negatively charged, and therefore like-charged molecules will exhibit repulsive forces. The isoelectric point is significant in protein purification because it represents the pH where solubility is typically minimal. Here, the protein isoelectric point signifies where mobility in an electro-focusing system is zero—and, in turn, the point where the protein will collect