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- IIT JAM
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Which of the following amino acid if added to an acidic solution will have buffer action on solution at the physiological ph? a. alanine b. glycine c. histidine
which of the following amino acid if added to an acidic solution will have buffer action on solution at the physiological pH? a. Alanine b. Glycine C. Histidine d. Arginine
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Anup Padwal Best Answer
Histidine is an important amino acid, because its side chain has a pKa near the physiological pH, so it can participate as a buffer of blood pH.
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Protein buffers in blood include haemoglobin (150g/l) and plasma proteins (70g/l). Buffering is by the imidazole group of the histidine residues which has a pKa of about 6.8. This is suitable for effective buffering at physiological pH. Haemoglobin is quantitatively about 6 times more important than the plasma proteins as it is present in about twice the concentration and contains about three times the number of histidine residues per molecule. Thus, the correct answer is option c.