profile-img
Ashutosh posted an Question
September 02, 2020 • 21:21 pm 30 points
  • IIT JAM
  • Biotechnology (BT)

Which one of the following is not true about klenow fragment? (a) it is a proteolytic cleavage product of dna polymerase-i (b) it has 5'-3' polymerase activity

Which one of the following is NOT TRUE about Klenow fragment? (a) It is a proteolytic cleavage product of DNA polymerase-I (b) It has 5'-3' polymerase activity (c) (d) It has 5'-3'exonuclease activity It has 3'-5' exonuclease activity

2 Answer(s) Answer Now
  • 0 Likes
  • 2 Comments
  • 0 Shares
  • Abhijeet Gaurav Best Answer

    Answer is (D). It doesn't have 5'-3' exonuclease activity. Because the 5' → 3' exonuclease activity of DNA polymerase I from E. coli makes it unsuitable for many applications, the Klenow fragment, which lacks this activity, can be very useful in research. The Klenow fragment is extremely useful for research-based tasks such as: Synthesis of double-stranded DNA from single-stranded templates Filling in receded 3' ends of DNA fragments to make 5' overhang blunt Digesting away protruding 3' overhangs Preparation of radioactive DNA probes

  • comment-profile-img>
    Dr. sharad agrawal best-answer

    4th option. it does not have 5 to 3 exonuclease activity.

whatsapp-btn

Do You Want Better RANK in Your Exam?

Start Your Preparations with Eduncle’s FREE Study Material

  • Updated Syllabus, Paper Pattern & Full Exam Details
  • Sample Theory of Most Important Topic
  • Model Test Paper with Detailed Solutions
  • Last 5 Years Question Papers & Answers