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Which one of the following is not true about klenow fragment? (a) it is a proteolytic cleavage product of dna polymerase-i (b) it has 5'-3' polymerase activity
Which one of the following is NOT TRUE about Klenow fragment? (a) It is a proteolytic cleavage product of DNA polymerase-I (b) It has 5'-3' polymerase activity (c) (d) It has 5'-3'exonuclease activity It has 3'-5' exonuclease activity
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Abhijeet Gaurav Best Answer
Answer is (D). It doesn't have 5'-3' exonuclease activity. Because the 5' → 3' exonuclease activity of DNA polymerase I from E. coli makes it unsuitable for many applications, the Klenow fragment, which lacks this activity, can be very useful in research. The Klenow fragment is extremely useful for research-based tasks such as: Synthesis of double-stranded DNA from single-stranded templates Filling in receded 3' ends of DNA fragments to make 5' overhang blunt Digesting away protruding 3' overhangs Preparation of radioactive DNA probes