Nisarg posted an Question
April 16, 2021 • 01:14 am 30 points
  • IIT JAM
  • Biotechnology (BT)

Why do we use klenow fragment in dna sequencing, why can't we use normal dna pol1!

why do we use klenow fragment in DNA sequencing, why can't we use normal DNA pol1!

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    Krishan k jakhad best-answer

    The Klenow fragment is a large protein fragment produced when DNA polymerase I from E. coli is enzymatically cleaved by the protease subtilisin... right now coming to ur question..why ?? Lacks or lost 5’ —> 3’ exonuclease activity think if it is present it will never extended...right

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